| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TMED1 recombinant protein (Position: P39-T227) was used as the immunogen for the TMED1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TMED1 Antibody / Transmembrane emp24 domain-containing protein 1 is a anti-TMED1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm, cell membrane.
Key elements and design rationale
- Target: TMED1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
TMED1 is encoded by the TMED1 gene located on human chromosome 19p13.3. The protein comprises a luminal GOLD (Golgi dynamics) domain, a single transmembrane helix, and a short cytoplasmic tail that interacts with coatomer components. TMED1 has been implicated in the trafficking of toll-like receptors and other immune-related proteins, highlighting its role in innate immune signaling. In the Golgi network, TMED1 contributes to the formation of transport vesicles and sorting of glycoproteins, making it a crucial element in secretory pathway organization.
Through use of the TMED1 antibody, researchers have identified strong expression in the Golgi apparatus and ER exit sites, where TMED1 co-localizes with other p24 family members such as TMED2, TMED4, and TMED10. Western blot typically reveals a band near 25-27 kDa corresponding to the full-length protein. Immunofluorescence labeling with this antibody provides a distinct perinuclear staining pattern consistent with Golgi localization. TMED1 expression has been linked to protein homeostasis, and its dysregulation may contribute to cellular stress responses and unfolded protein response activation.
Recent studies suggest TMED1's involvement in autophagy and viral protein processing, expanding its relevance beyond classical ER-Golgi transport. Knockdown or knockout experiments have shown altered secretion of select cytokines and membrane receptors, emphasizing its importance in vesicle biogenesis and immune communication.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
