| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TMED8 recombinant protein (Position: S34-S325) was used as the immunogen for the TMED8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TMED8 Antibody / Transmembrane emp24 domain-containing protein 8 is a anti-TMED8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: TMED8
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
TMED8 antibody identifies a protein that contributes to the maintenance of Golgi structure and trafficking efficiency. The p24 family proteins, including TMED8, function as cargo receptors that facilitate selective packaging of client proteins into COPII-coated vesicles at the ER membrane. They also participate in retrograde transport via COPI vesicles, ensuring the recycling of resident ER enzymes and chaperones. TMED8 specifically regulates the trafficking of glycoproteins and receptor complexes involved in cell signaling and immune response.
At the cellular level, TMED8 is localized predominantly to the endomembrane system, reflecting its function in the secretory pathway. Through its luminal GOLD domain, TMED8 mediates protein-protein interactions that stabilize cargo-receptor complexes, ensuring proper folding and export from the ER. Structural studies of the p24 family suggest that TMED8 forms oligomeric complexes with other p24 members, such as TMED2, TMED9, and TMED10, enhancing vesicular trafficking specificity.
Functionally, TMED8 has been implicated in immune regulation and inflammatory signaling. It is known to interact with components of the TGF-beta receptor pathway, influencing receptor stability and cell surface expression. Dysregulation of TMED8 expression may lead to impaired protein secretion, ER stress, and altered immune responses. In addition, emerging studies suggest a role for TMED8 in tumorigenesis, where aberrant expression correlates with altered cytokine signaling and cell adhesion. Gene expression analyses show TMED8 upregulation in certain cancers, including breast and colon carcinoma, linking it to enhanced secretory pathway activity.
The TMED8 gene�s chromosomal location on 14q11.2 places it near other trafficking-related genes, and its promoter region includes binding sites for transcription factors involved in ER stress response. Evolutionarily, TMED8 belongs to the TMED/p24 protein family, a conserved group essential for vesicular transport and Golgi organization. Defects in this pathway can lead to congenital disorders of glycosylation and impaired immune signaling.
Immunohistochemical staining using TMED8 antibody shows membrane and perinuclear localization consistent with Golgi and ER distribution, particularly in hepatocytes, epithelial cells, and macrophages. TMED8 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
