| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TMEFF1 recombinant protein (Position: K60-L331) was used as the immunogen for the TMEFF1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TMEFF1 Antibody / Transmembrane protein with EGF-like and two follistatin-like domains 1 is a anti-TMEFF1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TMEFF1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
TMEFF1 functions as a modulator of transforming growth factor beta (TGF-beta) and EGF signaling pathways. It binds heparin and cell-surface glycosaminoglycans, influencing receptor activation and downstream cascades. In neurons, TMEFF1 promotes survival and neurite extension, suggesting a role in brain development and maintenance. It has also been reported to exhibit tumor-suppressive properties by inhibiting EGF receptor signaling and cell proliferation in certain cancers.
Structurally, TMEFF1 contains an extracellular region with one epidermal growth factor (EGF)-like domain and two follistatin-like (FS) domains, a transmembrane helix, and a short cytoplasmic tail. These extracellular domains mediate ligand interactions, including potential binding with follistatin and growth factors, while the cytoplasmic region may transmit regulatory signals to intracellular effectors. TMEFF1 belongs to the follistatin domain protein family, known for regulating growth and differentiation through modulation of TGF-beta superfamily ligands.
TMEFF1 expression is most abundant in neurons, especially in the cerebellum, hippocampus, and olfactory bulb, indicating a role in neuronal connectivity and signaling. It is also detected in epithelial tissues of the prostate and testis. During development, TMEFF1 regulates neurogenesis and may act as a neuronal adhesion molecule. Co-localization studies show TMEFF1 associating with EGFR and ErbB2 at the plasma membrane, modulating their activation and downstream MAPK and PI3K-AKT pathways.
Dysregulation of TMEFF1 is implicated in cancer progression. Reduced expression correlates with poor prognosis in glioma, gastric, and colorectal cancers. Conversely, overexpression can suppress tumor growth by inhibiting proliferation and promoting apoptosis. The gene's promoter region is often hypermethylated in tumors, leading to transcriptional silencing. Pathway involvement includes TGF-beta signaling, EGFR signaling, and cell adhesion pathways.
TMEFF1 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
