| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TMEM232 recombinant protein (Position: D114-H598) was used as the immunogen for the TMEM232 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TMEM232 Antibody / Transmembrane protein 232 is a anti-TMEM232 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TMEM232
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, ELISA
Biological background
Structurally, TMEM232 contains hydrophobic alpha-helical domains typical of multipass membrane proteins, along with cytoplasmic loops that may participate in protein-protein interactions and intracellular signaling. The exact ligand or binding partners of TMEM232 remain under investigation, but functional genomics screens have implicated it in immune homeostasis and allergic disease susceptibility. Variants in the TMEM232 gene have been associated with atopic dermatitis and asthma, supporting its potential role in epithelial barrier integrity and immune response control.
TMEM232 expression appears to be regulated by inflammatory cytokines such as IL-4 and IL-13, consistent with its link to type 2 immune responses. It may contribute to the regulation of keratinocyte differentiation and tight junction assembly in epithelial tissues. In immune cells, TMEM232 has been detected in macrophages and lymphocytes, where it could participate in cell signaling pathways that mediate cytokine production and cell adhesion. Structural modeling predicts that TMEM232 may act as a membrane anchor for signaling complexes, similar to other poorly characterized transmembrane adaptors.
From a clinical perspective, genome-wide association studies (GWAS) have repeatedly identified polymorphisms in TMEM232 associated with allergic rhinitis, atopy, and asthma across multiple populations. The gene's proximity to cytokine regulatory regions and its expression pattern in airway epithelial cells support a role in respiratory immune function. Further studies suggest TMEM232 could modulate epithelial repair following inflammation or injury. Dysregulation of TMEM232 expression may contribute to chronic inflammatory diseases of the skin and respiratory tract.
Immunohistochemical analysis using TMEM232 antibody shows membrane localization in airway epithelium, keratinocytes, and lymphoid tissue. The antibody is useful for characterizing transmembrane signaling proteins and identifying novel immune regulatory pathways. TMEM232 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
