| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human TMEM43 was used as the immunogen for the TMEM43 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
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Overview
TMEM43 Antibody / Transmembrane protein 43 / LUMA is a anti-TMEM43 Rabbit antibody Recombinant Rabbit Monoclonal clone 30T30 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TMEM43
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30T30, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
Transmembrane protein 43 is highly conserved and associates with lamins and emerin within the nuclear envelope. Research using TMEM43 antibody has shown that it supports structural integrity of the nucleus and contributes to chromatin organization. TMEM43 interacts with protein complexes that link the nucleoskeleton to the cytoskeleton, enabling cells to respond to mechanical stimuli and maintain nuclear shape.
Studies with TMEM43 antibody have revealed that it plays essential roles in cardiac physiology. Mutations in the TMEM43 gene cause arrhythmogenic right ventricular cardiomyopathy, a hereditary disorder characterized by fibrofatty replacement of myocardium and increased risk of sudden cardiac death. Research into disease associated variants has demonstrated that loss of nuclear stability and altered signaling underlie this pathology. These findings highlight the importance of TMEM43 in maintaining cardiac health.
In addition to cardiovascular roles, Transmembrane protein 43 contributes to gene regulation. Research using TMEM43 antibody has indicated that nuclear envelope localization allows it to modulate transcription by influencing chromatin anchoring and positioning within the nucleus. These structural and regulatory functions make TMEM43 a multifunctional nuclear protein.
Dysregulation of TMEM43 is also linked to cancer. Studies with TMEM43 antibody have shown that changes in expression affect nuclear signaling pathways and may promote malignant transformation. Altered nuclear structure and mechanics, driven by TMEM43 dysfunction, contribute to abnormal growth and migration. These findings underscore the importance of nuclear envelope proteins in tumor biology.
TMEM43 antibody is commonly used in western blotting, immunofluorescence, and immunohistochemistry. Western blotting detects endogenous protein levels, immunofluorescence highlights localization to the nuclear envelope, and immunohistochemistry reveals tissue distribution in muscle and cardiac tissues. These applications make TMEM43 antibody valuable for nuclear biology and disease studies.
By providing validated TMEM43 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
