| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Meckelin was used as the immunogen for the TMEM67 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TMEM67 Antibody / Meckelin is a anti-TMEM67 Rabbit antibody Recombinant Rabbit Monoclonal clone 30T77 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TMEM67
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30T77, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, FACS
Biological background
TMEM67 antibody is widely applied in developmental biology, nephrology, and neurogenetics. By detecting meckelin, researchers can study how ciliary proteins regulate organ development and signaling. Ciliary dysfunction underlies a spectrum of disorders known as ciliopathies, linking TMEM67 research to human disease mechanisms.
Western blot assays detect meckelin in tissue lysates, while immunohistochemistry maps expression in kidney, brain, and liver. Immunofluorescence highlights punctate staining at primary cilia, consistent with its functional localization. These applications provide robust tools for examining ciliary protein biology.
Meckelin plays roles in regulating planar cell polarity, neuronal migration, and renal morphogenesis. Its dysfunction disrupts signaling and tissue organization, causing developmental defects and organ pathology. By applying TMEM67 antibody, scientists can investigate pathways linking ciliary function to embryogenesis, neurodevelopment, and kidney disease.
TMEM67 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
