| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | GFP (constitutively expressed), RFP (constitutively expressed), Hygromycin, Zeocin, Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
TMIGD2 (transmembrane and immunoglobulin domain-containing protein 2), also known as CD28H, is a co-stimulatory receptor expressed on naive T cells and natural killer cells. By engaging its ligand HHLA2 (B7-H7), TMIGD2 delivers positive signals that enhance T cell proliferation, cytokine production, and effector function. Co-stimulatory engagement promotes NFAT-dependent transcription downstream of the T cell receptor, so NFAT-driven reporters provide a quantitative readout of TMIGD2 signaling. As a member of the B7/CD28 family, the TMIGD2-HHLA2 axis is of growing interest in immuno-oncology, where it may be exploited to strengthen antitumor immune responses.
Product Description & Applications
The TMIGD2/NFAT Reporter Lentivirus is a two-vial immunotherapy reporter system for studying TMIGD2 co-stimulatory signaling. Vial 1 constitutively expresses human TMIGD2 with an antibiotic selection marker, while Vial 2 carries tandem NFAT response elements driving a dual reporter combining secreted Gaussia luciferase (GLuc) and a fluorescent protein (GFP or RFP). Sequential transduction and selection generate a dual-stable effector cell line that responds quantitatively to receptor engagement. Secreted GLuc accumulates in conditioned media for kinetic, lysis-free sampling, while the fluorescent reporter enables microscopy and flow cytometry. Applications include testing immune activation pathways, characterizing T cell responses, and evaluating co-stimulatory molecule function. Supplied as high-titer, VSV-G-pseudotyped third-generation lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation.
About This Product
This 2-vial immunotherapy reporter system consists of a Vial 1 Receptor Lentivirus encoding human TMIGD2 under a constitutive promoter with antibiotic selection, and a Vial 2 Reporter Lentivirus encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP-P2A-GLuc, GFP, RFP). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.
Secreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
