| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TMOD1 recombinant protein (Position: M1-V359) was used as the immunogen for the TMOD1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TMOD1 Antibody / Tropomodulin 1 is a anti-TMOD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: TMOD1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IP, FACS, ELISA
Biological background
In striated muscle, TMOD1 antibody identifies the isoform predominantly expressed in cardiac and slow skeletal muscle fibers, where it associates with tropomyosin and actin at the pointed end of thin filaments. TMOD1 plays an important role in sarcomere organization, length control, and muscle elasticity. Deficiency or mutation of TMOD1 disrupts sarcomere alignment, leading to myofibrillar disarray and cardiomyopathy phenotypes. In non-muscle cells, TMOD1 is involved in maintaining cortical actin networks, cell shape, and motility, particularly in erythrocytes where it preserves membrane mechanical stability and deformability.
TMOD1 antibody is used to study cytoskeletal architecture, cardiac muscle structure, and erythrocyte morphology. TMOD1 interacts with tropomyosin isoforms (TPM1-4) and capping proteins, coordinating actin filament dynamics during cell movement and myofibrillogenesis. In cardiac development, TMOD1 expression begins early in embryogenesis, highlighting its importance for contractile apparatus assembly. The TMOD1 gene, located on chromosome 9q22.33, encodes a 359-amino acid cytosolic protein that contains tropomyosin-binding and actin-capping domains. TMOD1 operates together with its isoforms TMOD2, TMOD3, and TMOD4, each showing tissue-specific distribution and regulatory mechanisms.
Altered TMOD1 expression has been linked to dilated cardiomyopathy, hereditary spherocytosis, and various muscle weakness disorders. Research also suggests TMOD1's involvement in cell adhesion, migration, and neuronal growth cone dynamics. Detection with TMOD1 antibody in immunostaining and western blot analyses allows detailed exploration of its role in filament organization and cell morphology under normal and disease conditions.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
