| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LAP2/TMPO recombinant protein (Position: N24-P672) was used as the immunogen for the TMPO antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TMPO Antibody / Thymopoietin is a anti-TMPO Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: TMPO
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, IP, FACS, ELISA
Biological background
During cell division, TMPO isoforms coordinate nuclear envelope breakdown and reassembly, interacting with Lamins A/C and chromatin-associated proteins such as BAF (Barrier-to-Autointegration Factor). The interaction between TMPO and Lamin A/C is essential for post-mitotic nuclear reformation and for anchoring chromatin to the nuclear periphery. Dysregulation of TMPO expression or mutation disrupts lamina organization, leading to nuclear morphology defects observed in laminopathies and premature aging syndromes. Beyond its structural roles, TMPO also influences cell proliferation and differentiation by modulating E2F-dependent transcription and histone deacetylase activity.
The TMPO antibody is used to detect Thymopoietin and its LAP2 isoforms in various experimental systems, including western blotting, immunofluorescence, and immunohistochemistry. It enables investigation of nuclear envelope integrity, chromatin positioning, and protein-protein interactions involving the nuclear lamina. In disease research, TMPO has been studied for its role in cancer cell cycle regulation and chromatin organization abnormalities. Its involvement in maintaining genomic stability makes it a valuable marker for nuclear envelope dynamics.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
