| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TMPRSS4 recombinant protein (Position: M1-K369) was used as the immunogen for the TMPRSS4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TMPRSS4 Antibody / Transmembrane protease serine 4 is a anti-TMPRSS4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TMPRSS4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
TMPRSS4 is expressed in epithelial tissues of the gastrointestinal tract, lung, and kidney, as well as in certain tumors. It participates in the proteolytic activation of signaling molecules and extracellular matrix remodeling. Elevated TMPRSS4 expression has been linked to cancer invasion and metastasis through the activation of integrin and epithelial-mesenchymal transition pathways. TMPRSS4 promotes cell motility and survival by enhancing FAK and Src kinase signaling, modulating cytoskeletal organization, and upregulating matrix metalloproteinases.
The TMPRSS4 antibody is a critical reagent for cancer, developmental, and epithelial biology research. Western blot analysis typically identifies a 50-60 kilodalton band corresponding to the cleaved active form, while immunohistochemistry reveals membrane and cytoplasmic staining in epithelial cells and tumor tissues. The antibody allows detection of TMPRSS4 expression in normal and malignant tissues, aiding studies of protease-mediated signaling and tumor microenvironment remodeling.
Beyond oncology, TMPRSS4 may regulate viral entry for certain coronaviruses and influenza strains by cleaving viral envelope glycoproteins. Its involvement in epithelial homeostasis and infection response makes it an emerging therapeutic target.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
