| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TNIP3 recombinant protein (Position: K30-P325) was used as the immunogen for the TNIP3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TNIP3 Antibody / TNFAIP3-interacting protein 3 is a anti-TNIP3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: TNIP3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Functionally, TNIP3 antibody identifies a 619-amino-acid adaptor protein that interacts with polyubiquitinated signaling intermediates in TNF and Toll-like receptor pathways. TNIP3 limits downstream signaling by promoting deubiquitination and degradation of key components such as TRAF6 and RIPK1. Through its regulatory activity, TNIP3 maintains immune balance and protects against chronic inflammation.
The TNIP3 gene is located on chromosome 4p15.2 and is expressed in immune tissues including spleen, lymph nodes, and macrophages. It is inducible by proinflammatory cytokines and interferons, functioning as part of a negative feedback loop to terminate NF-kappaB signaling. TNIP3 also contributes to cellular responses to oxidative stress and apoptosis.
Pathologically, dysregulation of TNIP3 has been linked to autoimmune and inflammatory diseases such as rheumatoid arthritis, psoriasis, and inflammatory bowel disease. Altered TNIP3 expression may enhance susceptibility to chronic immune activation. Research using TNIP3 antibody supports studies in inflammation, signal transduction, and immune regulation.
TNIP3 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect NF-kappaB regulatory proteins.
Structurally, TNFAIP3-interacting protein 3 contains multiple ubiquitin-binding domains that recognize polyubiquitin chains and mediate interaction with A20. This antibody facilitates investigation of TNIP3's function in inflammatory signaling and immune tolerance.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
