| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TNS3 recombinant protein (Position: Q592-E869) was used as the immunogen for the TNS3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TNS3 Antibody / Tensin 3 is a anti-TNS3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm, cell junctions.
Key elements and design rationale
- Target: TNS3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, IP, FACS, ELISA
Biological background
Structurally, Tensin-3 contains Src homology 2 (SH2) and phosphotyrosine-binding (PTB) domains that interact with phosphorylated tyrosine residues on focal adhesion proteins and with integrin cytoplasmic tails. Through these domains, TNS3 facilitates recruitment of signaling molecules, including Src family kinases, PI3K, and FAK, at adhesion sites. It also modulates Rho GTPase signaling, influencing actin organization and cell polarity.
The TNS3 antibody is widely used in cancer biology, cytoskeletal research, and cell signaling studies to investigate adhesion remodeling and integrin signaling. Western blot analysis detects a 150-160 kilodalton band corresponding to Tensin-3, while immunofluorescence reveals focal adhesion and cytoplasmic localization. This antibody supports studies examining adhesion turnover, migration, and mechanotransduction pathways in normal and malignant cells.
Tensin-3 expression is frequently altered in cancer, where it influences metastatic potential and invasion by regulating focal adhesion stability and actin remodeling. Overexpression promotes invasive behavior in several carcinoma types, while loss of function impairs migration and cell spreading. The TNS3 antibody provides a reliable reagent for exploring adhesion-mediated signaling and tumor cell motility.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
