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Histone H3 acetylation involves the addition of acetyl groups to lysine residues on histone proteins, which neutralizes their positive charge and relaxes chromatin structure, facilitating gene transcription, which are associated with DNA in chromatin. Histone acetylation is regulated by histone acetyltransferases (HATs), which add acetyl groups, and histone deacetylases (HDACs), which remove them. Histone H3 acetylation is tightly involved in cell cycle regulation, cell proliferation, and apoptosis. Disruption of histone acetylation balance in cancer cells, linked to abnormal transcription factor binding, promotes tumorigenesis, cancer progression, and metastasis. The Total Histone H3 Acetylation Detection Assay is designed to measure histone H3 acetylation in various mammalian cells, including human and mouse cells, as well as fresh and frozen tissues, cultured adherent cells, and suspension cells. This assay is simple, user-friendly, accurate, and fast. The measurement can be completed in 2.5 hours, with a recommended sample input of 5 ng to 2 µg per well. Alternative Name: H3ac
Alternative names: H3ac
Assay Principle
The Total Histone H3 Acetylation Detection Kit (Fluorometric) is a fluorometric immunoassay for the quantitative measurement of acetylated histone h3 in mammalian cells and tissues. Target histones are captured on a pre-coated microplate and detected with a modification-specific antibody, then quantified against a standard curve. The assay measures total histone h3 acetylation with a recommended sample input of 5 ng – 2 µg per well and can be completed in 2.5 hours.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (modification-specific antibody and enzyme/fluorophore conjugate), wash buffer, and developing/stop solutions. Refer to the product datasheet for the full component list and volumes.
Performance Data
| Assay Target | Total histone H3 acetylation |
|---|---|
| Analyte | Acetylated Histone H3 (total) |
| Assay Type | Fluorometric immunoassay |
| Detection Method | Fluorometric |
| Assay Time | 2.5 hours |
| Recommended Sample Input | 5 ng – 2 µg per well |
| Organism | Human, Mouse |
| Sample Compatibility | Mammalian cells (human, mouse); fresh & frozen tissues; adherent & suspension cells |
| Storage | Store the unopened kit at 2–8 °C. |
Safety & Handling
Can’t find the exact format you need? We can help you source or customize this total histone h3 acetylation assay — including fluorometric or alternative detection readout, plate/pack size (96-assay microplate), bulk packaging, or a validated protocol for your sample type (cells, tissue, adherent or suspension cultures). Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in contact shortly.
