| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TPBG recombinant protein (Position: E70-V420) was used as the immunogen for the TPBG antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TPBG Antibody / Trophoblast glycoprotein is a anti-TPBG Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TPBG
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
TPBG is encoded by the TPBG gene located on human chromosome 6q14.1. The mature protein is approximately 420 amino acids long and features an N-terminal signal peptide, several leucine-rich repeats, and an immunoglobulin-like domain in its extracellular region. It is anchored to the plasma membrane through a single transmembrane helix and contains a short cytoplasmic tail. Physiologically, TPBG modulates cell-cell interactions in placental development and embryo implantation, while pathologically, it is re-expressed in many epithelial tumors, where it contributes to epithelial-mesenchymal transition (EMT) and metastatic behavior.
The TPBG antibody is commonly used in immunohistochemistry and flow cytometry to identify tumor cells expressing the 5T4 antigen. Elevated TPBG expression has been reported in lung, ovarian, colorectal, and pancreatic cancers and is associated with poor prognosis and increased invasiveness. The protein influences Wnt/beta-catenin and CXCR4 signaling pathways, promoting migration and stem-like phenotypes. Western blot typically shows a broad band between 72-80 kDa, reflecting heavy glycosylation. Immunofluorescence microscopy reveals localization along the plasma membrane and at cell junctions.
In stem cell research, TPBG expression marks the transition from pluripotency to differentiation, making it a useful biomarker in embryonic stem cell studies. It modulates cytoskeletal dynamics and cell polarity through interactions with intracellular adaptors. Therapeutically, TPBG serves as a target for cancer immunotherapies, including antibody-drug conjugates and vaccines under clinical evaluation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
