| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TPCN2 recombinant protein (Position: L26-R752) was used as the immunogen for the TPCN2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TPCN2 Antibody / Two-pore channel 2 is a anti-TPCN2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TPCN2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
TPCN2 is widely expressed in kidney, liver, pancreas, and smooth muscle tissues, where it coordinates calcium-dependent signaling events. Activation of TPCN2 by NAADP triggers localized calcium release that modulates downstream pathways, including mTOR signaling, lysosomal exocytosis, and metabolic adaptation. Genetic studies have linked TPCN2 variants to pigmentation differences and hair color determination, highlighting its broader physiological influence. In neurons, TPCN2 regulates endolysosomal dynamics essential for synaptic vesicle recycling and signal transmission.
The TPCN2 antibody is a key reagent for cell signaling, membrane biology, and neurophysiology studies. Western blot analysis identifies a 75 kilodalton band corresponding to full-length TPCN2, while immunofluorescence reveals punctate staining consistent with lysosomal localization. The antibody enables detection of TPCN2 in subcellular fractionation and colocalization experiments with LAMP1 and Rab7 markers. Because endolysosomal calcium release impacts autophagy, metabolism, and exocytosis, this antibody supports research into pathways controlling intracellular trafficking and energy homeostasis.
Loss or dysregulation of TPCN2 impairs lysosomal function, alters autophagic flux, and contributes to disorders involving calcium signaling imbalance. Elevated expression has been associated with cancer cell invasiveness and altered vesicular pH homeostasis. The TPCN2 antibody allows researchers to evaluate how NAADP-dependent calcium signaling influences physiological and pathological processes.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
