| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TPD52 recombinant protein (Position: M1-L224) was used as the immunogen for the TPD52 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TPD52 Antibody / Tumor protein D52 is a anti-TPD52 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: TPD52
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
TPD52 is encoded by the TPD52 gene located on human chromosome 8q21.13. The protein is approximately 25 kilodaltons and characterized by a central coiled-coil domain that mediates homo- and hetero-oligomerization with other D52-like proteins (TPD52L1 and TPD52L2). This interaction facilitates vesicle docking and fusion processes within the Golgi network and secretory pathway. Expression is highest in epithelial tissues, immune cells, and tumors with high metabolic activity.
A TPD52 antibody detects a 25 kilodalton band in western blot analysis and reveals diffuse cytoplasmic and perinuclear staining in immunofluorescence assays. TPD52 associates with synaptotagmin-like proteins and small GTPases that control vesicle exocytosis, contributing to regulated secretion in endocrine and immune systems. Its upregulation enhances secretory granule release and cell motility, promoting oncogenic transformation and metastasis.
In cancer biology, TPD52 is implicated in cell-cycle progression, lipid storage, and energy metabolism. Overexpression increases tumorigenicity by stimulating AKT signaling and inhibiting apoptosis. Conversely, TPD52 depletion suppresses proliferation and sensitizes cells to chemotherapeutic stress. Clinical studies have identified elevated TPD52 expression in breast, ovarian, lung, and prostate cancers, often correlating with poor prognosis and high metastatic potential.
Beyond oncology, TPD52 participates in vesicle fusion during immune cell degranulation and insulin granule exocytosis, highlighting its role in both secretory and metabolic regulation. Phosphorylation by casein kinase 2 modulates its subcellular localization and protein-binding properties, linking signaling cues to vesicular transport efficiency.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
