| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TSN recombinant protein (Position: A16-D211) was used as the immunogen for the Translin antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Translin Antibody / TSN is a anti-TSN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Nucleus.
Key elements and design rationale
- Target: TSN
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
Structurally, Translin assembles into an octameric ring that binds nucleic acids in a sequence-independent manner. It contains a central RNA/DNA-binding domain and regions that mediate oligomerization and association with Trax. Together, Translin and Trax form the C3PO complex, which promotes RNA-induced silencing by accelerating siRNA duplex unwinding. Translin belongs to the Translin family of RNA-binding proteins that regulate post-transcriptional processes across multiple tissues.
Functionally, Translin acts in mRNA transport, localization, and stabilization. In neurons, it regulates axonal mRNA transport and local translation critical for synaptic plasticity. It also participates in DNA double-strand break repair and chromosomal translocation control, linking RNA metabolism to genome maintenance. Through the C3PO complex, Translin contributes to RNAi efficiency and microRNA (miRNA) processing, ensuring proper post-transcriptional gene regulation. Known binding partners include TSNAX (Trax), AGO2, and Dicer, all involved in RNA interference and processing.
Translin expression is developmentally regulated, peaking during neuronal maturation and spermatogenesis. In germ cells, it contributes to mRNA trafficking essential for gametogenesis. In immune cells, it regulates cytokine mRNA stability and turnover. Dysregulation of Translin has been linked to neurodevelopmental disorders and cancers, where aberrant RNA regulation and chromosomal rearrangements contribute to disease progression. Pathway associations include RNA transport, RNA interference, and DNA damage repair signaling.
The Translin antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
