| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TRIM11 recombinant protein (Position: M1-R336) was used as the immunogen for the TRIM11 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TRIM11 Antibody / Tripartite motif-containing protein 11 is a anti-TRIM11 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: TRIM11
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Encoded by the TRIM11 gene located on human chromosome 1q42.13, the protein is composed of approximately 465 amino acids and localizes predominantly to the cytoplasm, although nuclear accumulation occurs under specific stress conditions. Functionally, TRIM11 mediates the ubiquitination and degradation of misfolded or aggregated proteins, thereby maintaining neuronal homeostasis. It directly interacts with substrates such as Humanin, a neuroprotective peptide, and acts as a negative regulator of stress-induced signaling. Through its ubiquitin ligase activity, TRIM11 contributes to proteasome-mediated clearance of abnormal proteins, preventing cytotoxic accumulation observed in neurodegenerative diseases like Alzheimer's and Parkinson's.
The TRIM11 antibody is also valuable for studying tumorigenesis, as elevated TRIM11 expression has been reported in glioma, breast, and colorectal cancers. It promotes cell proliferation and migration by targeting tumor suppressors for degradation and by activating signaling pathways such as PI3K/AKT and ERK/MAPK. Additionally, TRIM11 can suppress interferon-stimulated gene expression, indicating a regulatory role in antiviral immunity. Western blot analyses using this antibody typically reveal a band near 52-55 kDa, corresponding to the full-length protein. Immunofluorescence assays show diffuse cytoplasmic localization with punctate distribution, consistent with its role in protein turnover and ubiquitin signaling.
At the molecular level, TRIM11 associates with proteasomal subunits and chaperone complexes, enhancing clearance of misfolded proteins generated during oxidative stress or viral infection. Knockdown studies reveal increased accumulation of aggregation-prone proteins and elevated sensitivity to stress-induced apoptosis, demonstrating TRIM11's role as a cellular quality control factor. The protein also regulates transcriptional activity by modulating the degradation of transcriptional co-repressors and signaling intermediates.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
