| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TRIM2 recombinant protein (Position: Q78-E398) was used as the immunogen for the TRIM2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TRIM2 Antibody / Tripartite motif-containing protein 2 is a anti-TRIM2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Mitochondria, ER.
Key elements and design rationale
- Target: TRIM2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
TRIM2 is encoded by the TRIM2 gene located on human chromosome 4q31.3. The protein is approximately 744 amino acids long and contains a RING finger domain, two B-boxes, a coiled-coil region, and a C-terminal filamin-binding NHL repeat domain. TRIM2 localizes to axons, growth cones, and perinuclear regions, reflecting its role in neuronal trafficking and cytoskeletal regulation.
The TRIM2 antibody detects a 90 kilodalton band by western blot and reveals filamentous cytoplasmic staining under immunofluorescence microscopy. TRIM2 ubiquitinates neurofilament light chain (NEFL) to control filament organization and turnover, maintaining axonal integrity and preventing neurodegenerative aggregation. It also regulates apoptosis by targeting proapoptotic proteins for degradation and interacts with signaling pathways involved in neuronal survival.
Mutations in TRIM2 cause hereditary axonal neuropathy and neurodegenerative disease characterized by axon swelling and degeneration. Dysregulation of TRIM2 expression has also been linked to gliomas and other cancers, where altered ubiquitination affects cytoskeletal dynamics and invasion. In developing neurons, TRIM2 coordinates cytoskeletal remodeling during axon guidance and branching.
Because of its key role in ubiquitin-mediated regulation of the neuronal cytoskeleton, TRIM2 serves as an essential molecule for understanding axonal maintenance and neuroprotection.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
