| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TRIM31 recombinant protein (Position: R72-E335) was used as the immunogen for the TRIM31 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TRIM31 Antibody / Tripartite motif-containing protein 31 is a anti-TRIM31 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TRIM31
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
TRIM31 is encoded by the TRIM31 gene on human chromosome 6p22.1. The protein is approximately 478 amino acids long and localizes to the cytoplasm and mitochondria. TRIM31 acts as an E3 ubiquitin ligase that targets specific signaling intermediates for degradation or activation, thus modulating innate immune pathways such as RIG-I and MAVS. Through ubiquitination, TRIM31 promotes antiviral signaling by facilitating MAVS aggregation and interferon production.
The TRIM31 antibody detects a 55 kilodalton protein by western blot and demonstrates cytoplasmic punctate staining. In addition to its role in antiviral defense, TRIM31 regulates inflammasome activation and apoptosis. It ubiquitinates NLRP3 and p53, thereby controlling inflammatory and apoptotic responses. Dysregulation of TRIM31 leads to chronic inflammation and has been implicated in autoimmune diseases and metabolic disorders.
In cancer, TRIM31 can function as both an oncogene and tumor suppressor depending on cellular context. Overexpression of TRIM31 promotes proliferation and invasion in gastric and pancreatic cancers, whereas loss of TRIM31 contributes to tumorigenesis through impaired apoptosis regulation. TRIM31 expression is also linked to mitochondrial quality control via regulation of mitophagy, emphasizing its multifaceted cellular functions.
As a key E3 ligase coordinating immune, inflammatory, and metabolic pathways, TRIM31 represents a versatile model for studying ubiquitin signaling networks.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
