| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TRIM32 recombinant protein (Position: S216-R586) was used as the immunogen for the TRIM32 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TRIM32 Antibody / Tripartite motif-containing protein 32 is a anti-TRIM32 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TRIM32
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
TRIM32 is encoded by the TRIM32 gene located on human chromosome 9q33.1. The protein is approximately 653 amino acids long and contains a RING domain, one or two B-box domains, and a coiled-coil region that mediates protein-protein interactions, followed by a C-terminal NHL repeat domain responsible for substrate recognition. TRIM32 localizes to the cytoplasm, nucleus, and occasionally to cytoskeletal structures, depending on cell type and activity state.
The TRIM32 antibody detects an 80 kilodalton protein by western blot and shows punctate cytoplasmic staining under immunofluorescence microscopy. TRIM32 functions as an E3 ubiquitin ligase that targets a variety of substrates involved in differentiation, including actin regulators, transcription factors, and signaling molecules. In skeletal muscle, TRIM32 promotes myogenesis by ubiquitinating inhibitors of differentiation such as NDRG2 and MyoD regulators. In neurons, TRIM32 modulates synaptic plasticity and axonal growth by regulating cytoskeletal remodeling and degradation of synaptic proteins.
Mutations in TRIM32 cause Limb-Girdle Muscular Dystrophy type 2H and Bardet-Biedl syndrome, disorders characterized by muscle weakness, retinal degeneration, and developmental defects. These mutations often impair E3 ligase activity or disrupt substrate binding, leading to accumulation of misfolded or damaged proteins. TRIM32 is also implicated in tumorigenesis, acting as either a tumor suppressor or oncogene depending on cellular context. Its ubiquitination of MYC and p53 pathway components links it directly to cancer cell proliferation and apoptosis.
Because TRIM32 integrates ubiquitin signaling with cellular differentiation and homeostasis, it serves as an essential node in protein quality control and tissue development.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
