| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TRIM39 recombinant protein (Position: K37-K490) was used as the immunogen for the TRIM39 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TRIM39 Antibody / Tripartite motif-containing protein 39 is a anti-TRIM39 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: TRIM39
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
TRIM39 is encoded by the TRIM39 gene on human chromosome 6p22.1. The protein localizes to both cytoplasmic and nuclear compartments and serves as a negative regulator of the anaphase-promoting complex/cyclosome (APC/C), a ubiquitin ligase critical for mitotic progression. Through this inhibition, TRIM39 stabilizes APC/C substrates and contributes to cell cycle control. It also mediates ubiquitination of transcription factors such as NFATc3 and c-Jun, thereby influencing apoptosis and immune signaling. TRIM39 expression is tightly regulated during stress, infection, and DNA damage.
Western blot using the TRIM39 antibody typically reveals an ~60 kilodalton band. Immunofluorescence demonstrates punctate cytoplasmic staining consistent with TRIM family bodies involved in ubiquitin signaling. Functional assays show that TRIM39 enhances cell proliferation by targeting pro-apoptotic proteins for degradation but can also promote cell death under specific conditions, highlighting its dual regulatory nature. In cancer, TRIM39 overexpression has been observed in colorectal and hepatic tumors, where it modulates p53 activity and supports cell survival. In contrast, downregulation impairs growth and increases sensitivity to stress-induced apoptosis.
TRIM39 also plays roles in antiviral responses, acting as a modulator of type I interferon signaling. By ubiquitinating immune regulators, it fine-tunes cytokine production and antiviral defense. Beyond its role in signaling, TRIM39 may interact with chromatin-associated proteins, influencing gene transcription and epigenetic regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
