| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TRIM40 recombinant protein (Position: M1-L258) was used as the immunogen for the TRIM40 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TRIM40 Antibody / Tripartite motif-containing protein 40 is a anti-TRIM40 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: TRIM40
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Biochemical studies have demonstrated that TRIM40 mediates ubiquitination and degradation of components within the NF-kappaB pathway, including IKK gamma (NEMO), thereby dampening inflammatory signaling. It is expressed predominantly in gastrointestinal epithelial cells and immune tissues, where it contributes to immune tolerance and inflammation control. TRIM40 also regulates Rho GTPase signaling, maintaining cytoskeletal integrity and epithelial barrier function. Reduced expression or dysregulation of TRIM40 has been associated with inflammatory bowel disease, colorectal cancer, and autoimmune conditions, suggesting its role as a protective modulator of mucosal immunity.
Located on chromosome 6p21.33 within the MHC class I region, the TRIM40 gene is inducible by cytokines and microbial stimuli. The protein's E3 ligase activity enables it to tag target proteins for proteasomal degradation, ensuring tight control of inflammatory pathways. In addition to immune regulation, TRIM40 may influence oncogenic processes by modulating NF-kappaB-driven transcription of genes related to proliferation and survival. Overexpression studies indicate that TRIM40 stabilizes cytoskeletal structures and promotes cell adhesion, counteracting tumor cell invasiveness.
Immunostaining using TRIM40 antibody demonstrates cytoplasmic localization in intestinal epithelium and lymphoid tissues. The antibody serves as a valuable reagent for exploring immune signaling, epithelial cell regulation, and tumor suppression mechanisms. TRIM40 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
