| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TRIM44 recombinant protein (Position: M1-E332) was used as the immunogen for the TRIM44 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TRIM44 Antibody / Tripartite motif-containing protein 44 is a anti-TRIM44 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TRIM44
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, TRIM44 is a 512-amino-acid cytoplasmic protein of approximately 57 kilodaltons containing a zinc-binding B-box and coiled-coil domains typical of TRIM proteins. Unlike many family members, TRIM44 lacks a canonical RING finger domain but instead contains a zinc metalloprotease-like region that mediates protein stabilization rather than degradation. This structural divergence enables TRIM44 to protect target proteins from proteasomal degradation, thereby modulating multiple signaling cascades including NF-?B and PI3K/AKT pathways.
The TRIM44 antibody is widely used in cancer biology, immunology, and cell signaling research to study ubiquitin-dependent protein regulation, tumor progression, and cellular stress response. Western blot analysis detects a 57 kilodalton band corresponding to TRIM44, while immunofluorescence shows cytoplasmic and perinuclear staining, reflecting its role in signaling and vesicular trafficking. This antibody provides a dependable reagent for investigating the molecular mechanisms by which TRIM44 regulates protein turnover and signal transduction.
Functionally, TRIM44 stabilizes key signaling proteins including mTOR and NF-?B regulators, promoting survival under stress conditions such as oxidative damage or nutrient deprivation. Overexpression of TRIM44 has been observed in multiple cancers including gastric, lung, and breast carcinomas, where it enhances proliferation, migration, and epithelial-mesenchymal transition (EMT). Conversely, its depletion sensitizes cells to apoptosis and autophagic degradation. The TRIM44 antibody supports research exploring ubiquitin signaling networks, autophagy regulation, and cancer-associated proteostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
