| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TRIM59 recombinant protein (Position: E71-S313) was used as the immunogen for the TRIM59 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TRIM59 Antibody / Tripartite motif-containing protein 59 is a anti-TRIM59 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: ER.
Key elements and design rationale
- Target: TRIM59
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
TRIM59 is encoded by the TRIM59 gene on human chromosome 3q25.33. The protein localizes mainly to the cytoplasm but can translocate to the nucleus under specific signaling conditions. It interacts with multiple oncogenic and tumor-suppressive pathways, including p53, STAT3, and Ras-mediated cascades. Overexpression of TRIM59 has been documented in various cancers such as gastric, lung, and breast carcinoma, where it promotes cell proliferation, invasion, and resistance to apoptosis.
Studies using the TRIM59 antibody have revealed that TRIM59 acts as an oncogenic factor by promoting ubiquitin-dependent degradation of p53, thereby suppressing apoptosis and enhancing tumor growth. Additionally, TRIM59 participates in innate immune signaling by modulating the STING and NF-kB pathways, influencing cytokine production during viral infection. Western blot analysis typically shows a band at approximately 42-48 kDa. Immunofluorescence using this antibody reveals diffuse cytoplasmic staining, occasionally enriched in perinuclear compartments.
TRIM59 functions as part of a larger regulatory network controlling inflammation and autophagy. It has been associated with epigenetic modulation through interactions with chromatin-remodeling factors, affecting transcriptional activation of oncogenes. In cancer models, TRIM59 depletion suppresses tumor cell migration and enhances p53-dependent growth inhibition.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
