| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TRIP6 recombinant protein (Position: P8-C476) was used as the immunogen for the TRIP6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TRIP6 Antibody / Thyroid receptor-interacting protein 6 is a anti-TRIP6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TRIP6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IP, FACS, ELISA
Biological background
TRIP6 is encoded by the TRIP6 gene located on human chromosome 7q22.1. The protein is approximately 476 amino acids long and contains three LIM domains that mediate interactions with actin-associated proteins, kinases, and transcriptional regulators. TRIP6 localizes to focal adhesions, the cytoplasm, and the nucleus, reflecting its multifunctional role in signaling and structural regulation.
The TRIP6 antibody detects a 50 kilodalton band by western blot and shows strong focal adhesion staining under immunofluorescence. TRIP6 interacts with proteins such as paxillin, LPP, and integrin-linked kinase to regulate actin remodeling and cell migration. It also functions as a coactivator of transcription factors, including NF-kappaB and AP-1, linking mechanical and inflammatory signaling pathways.
In cancer, TRIP6 is frequently overexpressed and contributes to tumor cell migration, invasion, and survival. It mediates integrin and growth factor signaling, promoting metastasis through focal adhesion turnover and epithelial-to-mesenchymal transition. Conversely, in certain cell types, TRIP6 may act as a negative regulator of apoptosis and inflammation, demonstrating context-dependent roles.
As a versatile adaptor integrating cytoskeletal and transcriptional functions, TRIP6 is an important regulator of cellular mechanics and gene expression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
