| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TSR1 recombinant protein (Position: D224-V723) was used as the immunogen for the TSR1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TSR1 Antibody / Pre-rRNA-processing protein TSR1 is a anti-TSR1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TSR1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, IP, FACS, ELISA
Biological background
TSR1 is encoded by the TSR1 gene located on human chromosome 17q25.3. The protein is approximately 88 kilodaltons and conserved across eukaryotes, underscoring its critical role in ribosome biogenesis. TSR1 associates with pre-ribosomal particles in the nucleolus and cytoplasm and functions during the transition of 90S to 40S ribosomal precursors. It acts as a structural scaffold that stabilizes pre-rRNA intermediates and recruits processing enzymes necessary for final 18S rRNA cleavage.
The TSR1 antibody typically detects an 88-90 kilodalton band on western blot and is useful for immunofluorescence studies showing nucleolar and cytoplasmic localization. Knockdown or mutation of TSR1 disrupts ribosome biogenesis, leading to impaired protein synthesis and activation of nucleolar stress pathways such as p53 stabilization. As a core assembly factor, TSR1 interacts with RIO kinases, NOB1, and other late-acting ribosome maturation proteins.
In addition to its canonical role in ribosome formation, TSR1 may contribute to cell cycle progression and proliferation through its control of global translation. Aberrant expression of TSR1 has been observed in several cancers, suggesting a link between elevated ribosome production and tumorigenesis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
