| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TTBK1 recombinant protein (Position: D285-A956) was used as the immunogen for the TTBK1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TTBK1 Antibody / Tau-tubulin kinase 1 is a anti-TTBK1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: TTBK1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, TTBK1 antibody identifies a 1,326-amino-acid cytoplasmic and axonal kinase that phosphorylates tau at multiple sites, including those associated with Alzheimer's disease pathology. TTBK1 regulates microtubule nucleation and cilia disassembly, contributing to neuronal morphogenesis and signaling. It is primarily expressed in the cerebral cortex, hippocampus, and cerebellum, where it modulates cytoskeletal organization and synaptic plasticity.
The TTBK1 gene is located on chromosome 6p21.1 and is expressed predominantly in neurons. Expression is upregulated during axonal outgrowth and differentiation, linking kinase activity to neural development. TTBK1 functions within signaling cascades involving CDK5 and GSK3beta, coordinating tau modification and cytoskeletal remodeling in neurons.
Pathologically, aberrant activation or overexpression of TTBK1 contributes to tau hyperphosphorylation, neurofibrillary tangle formation, and neuronal death in Alzheimer's disease and other tauopathies. Mutations or altered regulation may also affect primary cilia dynamics and neuronal polarity. Research using TTBK1 antibody supports studies in neurodegeneration, kinase signaling, and cytoskeletal biology.
TTBK1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect neuronal kinases.
Structurally, Tau-tubulin kinase 1 contains an N-terminal kinase domain with conserved ATP-binding and catalytic residues, and a C-terminal regulatory region that mediates substrate specificity and microtubule association. TTBK1 undergoes autophosphorylation and dynamic localization between cytoplasm and axon terminals. This antibody enables detailed examination of TTBK1's role in neuronal architecture and tau-related pathology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
