| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TTLL1 recombinant protein (Position: V26-Q402) was used as the immunogen for the TTLL1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TTLL1 Antibody / Tubulin-tyrosine ligase-like protein 1 is a anti-TTLL1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: TTLL1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
Tubulin-tyrosine ligase-like protein 1 belongs to the tubulin tyrosine ligase-like family, a group of enzymes that mediate tubulin polyglutamylation and polyglycylation. It primarily acts on alpha-tubulin within stable microtubule arrays such as those found in neurons and cilia. The TTLL1 antibody supports visualization of enzyme expression in neuronal soma and axons, reflecting its enrichment in brain tissue and importance for neural function. Loss of TTLL1 leads to reduced tubulin polyglutamylation, defective neuronal transport, and abnormal ciliary movement.
Polyglutamylation modulates the binding of microtubule-associated proteins (MAPs) and molecular motors including kinesin and dynein. The TTLL1 antibody provides a valuable reagent for analyzing this regulatory layer of cytoskeletal control. Dysregulation of TTLL1 activity contributes to neurodegenerative and ciliopathy phenotypes due to altered microtubule organization. TTLL1-dependent modification also influences mitotic spindle assembly and chromosome segregation.
Tubulin-tyrosine ligase-like protein 1 localizes to the cytoplasm and is often concentrated at centrosomes and ciliary bases. The TTLL1 antibody supports localization studies revealing its participation in microtubule-anchoring regions. Mutations in TTLL1 have been associated with human developmental disorders involving cerebellar atrophy and cognitive delay, reinforcing its essential role in neuronal microtubule regulation.
The TTLL1 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, yielding distinct cytoplasmic and centrosomal staining.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
