| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TTLL5 recombinant protein (Position: R111-E1015) was used as the immunogen for the TTLL5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TTLL5 Antibody / Tubulin tyrosine ligase-like protein 5 is a anti-TTLL5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: TTLL5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
TTLL5 is expressed in multiple tissues, including retina, testis, and brain, where it supports specialized microtubule structures such as photoreceptor connecting cilia and sperm flagella. In photoreceptor cells, TTLL5 regulates the glutamylation of ciliary axonemes and outer segment proteins critical for vision. Mutations in TTLL5 cause inherited retinal dystrophies, including cone-rod dystrophy and Leber congenital amaurosis, due to defective ciliary transport and photoreceptor degeneration. In spermatozoa, TTLL5 is essential for flagellar stability and motility, and its absence leads to asthenozoospermia and male infertility.
The TTLL5 antibody is widely used in cell biology, neurobiology, and reproductive research to study microtubule post-translational modification and ciliary biology. Western blot analysis identifies a 130 kilodalton band corresponding to the full-length enzyme, while immunofluorescence reveals localization along ciliary and cytoplasmic microtubules. Researchers employ this antibody to investigate TTLL5-dependent pathways controlling axonemal function and intracellular transport. Dysregulation of tubulin glutamylation has been implicated in neurodegenerative diseases and ciliopathies, making TTLL5 an important molecular marker for cytoskeletal regulation.
Functionally, TTLL5 acts with other TTLL family members to maintain the dynamic balance between glutamylation and deglutamylation, which modulates motor protein activity and vesicular transport. The TTLL5 antibody facilitates the study of these processes and enables precise mapping of TTLL5 expression across tissues.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
