| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TWSG1 recombinant protein (Position: E22-A199) was used as the immunogen for the TWSG1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TWSG1 Antibody / Twisted gastrulation protein homolog 1 is a anti-TWSG1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: TWSG1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
Functionally, TWSG1 antibody identifies a 223-amino-acid secreted protein that forms complexes with Chordin and BMPs (including BMP2, BMP4, and BMP7), modulating the bioavailability and receptor binding of BMP ligands. TWSG1 can either inhibit or enhance BMP signaling depending on tissue context, concentration, and association with proteases such as tolloid metalloproteinases. It contributes to dorsoventral patterning, skeletal formation, and hematopoietic stem cell maintenance by maintaining optimal BMP activity levels during development.
The TWSG1 gene is located on chromosome 18p11.21 and is expressed in a variety of embryonic and adult tissues including brain, kidney, bone, and reproductive organs. Expression is dynamically regulated by growth factors, Wnt signaling, and retinoic acid, ensuring coordinated morphogenic signaling across developing tissues.
Pathologically, mutations or reduced expression of TWSG1 cause craniofacial malformations and developmental defects due to disrupted BMP gradients. Altered TWSG1 signaling has also been implicated in cancer, fibrosis, and abnormal bone remodeling. Overexpression may contribute to tumor progression by influencing extracellular matrix deposition and epithelial-mesenchymal transition. Research using TWSG1 antibody supports studies in developmental biology, morphogen signaling, and cancer research.
TWSG1 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect secreted morphogen modulators.
Structurally, Twisted gastrulation protein homolog 1 contains two cysteine-rich domains stabilized by disulfide bonds, essential for ligand binding and signaling modulation. These domains enable interactions with both BMPs and their inhibitors, allowing TWSG1 to function as a molecular switch in morphogen networks. This antibody enables exploration of TWSG1's role in extracellular signaling, organ patterning, and skeletal formation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
