| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TXNRD1 recombinant protein (Position: Y93-Y326) was used as the immunogen for the TXNRD1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TXNRD1 Antibody / Thioredoxin reductase 1 is a anti-TXNRD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: TXNRD1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, TXNRD1 antibody identifies a 654-amino-acid cytosolic enzyme composed of an FAD-binding domain, NADPH-binding domain, and a selenocysteine-containing active site motif (GCUG). TXNRD1 reduces oxidized thioredoxin, which in turn regenerates reduced cysteine residues in target proteins, maintaining redox homeostasis. It also directly reduces lipid hydroperoxides, hydrogen peroxide, and other disulfide-containing molecules, contributing to cellular antioxidant capacity.
The TXNRD1 gene is located on chromosome 12q23.3 and encodes the cytosolic isoform of thioredoxin reductase. TXNRD1 expression is regulated by the transcription factor NRF2 through the antioxidant response element (ARE) under oxidative or electrophilic stress. Its activity is essential for maintaining a reducing intracellular environment, supporting cell survival, and modulating signaling pathways such as apoptosis and proliferation.
In redox biology, TXNRD1 plays a dual role: it protects cells from oxidative stress yet also supports cancer cell survival under high metabolic demand. Elevated TXNRD1 expression is frequently observed in tumors, where it promotes resistance to chemotherapeutic agents and radiotherapy. Conversely, TXNRD1 inhibition induces oxidative stress, mitochondrial dysfunction, and apoptosis, making it a therapeutic target for cancer and inflammatory diseases.
Beyond oncology, TXNRD1 contributes to immune function, lipid metabolism, and cardiovascular health. Its deficiency leads to increased susceptibility to oxidative damage, accelerated aging, and metabolic imbalance. In experimental models, altered TXNRD1 expression affects insulin sensitivity and lipid peroxidation levels, linking it to metabolic and neurodegenerative disorders.
TXNRD1 antibody is widely used in redox biology, metabolism, and cancer research. It is suitable for western blotting, immunohistochemistry, and enzymatic assays to monitor TXNRD1 expression and activity. This antibody supports investigations into antioxidant defense, redox signaling, and selenoprotein regulation. In pharmacology, it helps evaluate TXNRD1 as a biomarker of oxidative stress and drug response.
Structurally, TXNRD1 forms a homodimer with active sites on each subunit and utilizes FAD and NADPH cofactors for electron transfer. Its C-terminal selenocysteine residue is critical for catalytic turnover.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
