| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PD-ECGF/TYMP recombinant protein (Position: P13-Q482) was used as the immunogen for the TYMP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TYMP Antibody / Thymidine phosphorylase / PD-ECGF is a anti-TYMP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: TYMP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IP, FACS, ELISA
Biological background
As a key enzyme in pyrimidine salvage metabolism, TYMP maintains nucleotide pool balance for DNA synthesis and repair. Beyond its catalytic role, extracellular TYMP functions as PD-ECGF, promoting endothelial cell migration, angiogenesis, and wound healing. TYMP activity increases during hypoxia and tissue regeneration, facilitating the formation of new capillaries in response to injury or ischemia. Co-localization studies show TYMP associating with endothelial integrins and extracellular matrix components in angiogenic tissues.
Structurally, TYMP forms a homodimer with each subunit containing a thymidine-binding pocket and phosphate-binding residues essential for catalysis. It belongs to the thymidine phosphorylase family of pyrimidine salvage enzymes. The enzyme's catalytic activity also generates deoxyribose sugars that act as angiogenic mediators, linking metabolism to vascular signaling. TYMP interacts with molecules such as integrin alphavbeta3 and extracellular matrix proteins to enhance endothelial cell adhesion and migration.
Functionally, TYMP contributes to multiple biological processes, including angiogenesis, platelet activation, and oxidative stress response. Its expression is regulated by hypoxia-inducible factor 1-alpha (HIF-1alpha), cytokines, and growth factors such as VEGF and TNF-alpha. In cancer, TYMP expression is upregulated in tumor-associated macrophages and endothelial cells, promoting neovascularization and tumor progression. However, TYMP also exhibits cytotoxic effects in thymidine phosphorylase-deficient conditions by accumulating toxic thymidine metabolites, as seen in mitochondrial neurogastrointestinal encephalopathy (MNGIE).
Dysregulation of TYMP is clinically significant. Loss-of-function mutations cause MNGIE, a rare mitochondrial disorder characterized by gastrointestinal dysmotility, neuropathy, and leukoencephalopathy. Overexpression of TYMP is associated with poor prognosis in several cancers, including colorectal, breast, and gastric carcinoma, where it drives angiogenesis and tumor growth. Pathway involvement includes pyrimidine salvage metabolism, hypoxia response, and VEGF-mediated angiogenic signaling. In regenerative medicine, TYMP serves as a biomarker and target for promoting vascular repair and wound healing.
Immunohistochemical staining using TYMP antibody shows cytoplasmic and extracellular localization in endothelial and stromal cells. The TYMP antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
