| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human TYRP1 recombinant protein (Position: R131-V537) was used as the immunogen for the TYRP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TYRP1 Antibody / Tyrosinase-related protein 1 is a anti-TYRP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Melanosome).
Key elements and design rationale
- Target: TYRP1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
Functionally, TYRP1 antibody identifies a 537-amino-acid type I transmembrane glycoprotein localized to melanosomal membranes. TYRP1 contributes to the oxidative conversion of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) during eumelanin synthesis and stabilizes the activity of tyrosinase through protein-protein interactions. It plays a central role in regulating pigmentation intensity, melanosome biogenesis, and melanocyte survival. In addition, TYRP1 acts as an antioxidant enzyme, protecting pigment cells from oxidative stress generated during melanin production.
The TYRP1 gene is located on chromosome 9p23 and is specifically expressed in melanocytes and retinal pigment epithelial cells. Its expression is tightly regulated by the microphthalmia-associated transcription factor (MITF), which coordinates the transcriptional program of melanogenesis. TYRP1 expression levels and genetic polymorphisms contribute to pigment variation among populations and species.
Pathologically, mutations in TYRP1 cause oculocutaneous albinism type 3 (OCA3), characterized by reduced pigmentation and visual abnormalities. Altered TYRP1 expression has also been associated with melanoma progression, where it influences tumor cell differentiation and immune recognition. Research using TYRP1 antibody supports studies in pigmentation biology, melanosome formation, and melanoma immunology.
TYRP1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect melanosomal proteins.
Structurally, Tyrosinase-related protein 1 contains a luminal catalytic domain with two copper-binding sites and multiple glycosylation motifs that ensure enzymatic stability and trafficking. Its C-terminal transmembrane region anchors it to melanosomal membranes, while its N-terminal signal sequence directs localization to the secretory pathway. This antibody enables investigation of TYRP1's role in melanin biosynthesis, pigment cell regulation, and disease mechanisms affecting coloration.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
