| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Actin, alpha cardiac muscle 1;Alpha-cardiac actin;Actin, alpha skeletal muscle;Alpha-actin-1;Actin, gamma-enteric smooth muscle;Alpha-actin-3;Gamma-2-actin;Smooth muscle gamma-actin |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Synthesized peptide derived from human Actin, Muscle Specific AA range: 2-50 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Actin, Muscle Specific (ABT-MSA) Mouse Monoclonal Antibody (WM1283) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Actin_Muscle Specific. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat, Monkey, Bovine, Pig, Chicken.
Validated Applications
| Target | Actin_Muscle Specific |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT-MSA |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Monkey, Bovine, Pig, Chicken |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize proteins from smooth muscle, myocardium and skeletal muscle α actin and smooth muscle derived γ actin, and β actin or non smooth muscle derived γ actin does not respond. |
| Molecular Weight | Observed: 42 kDa |
| UniProt / Swiss-Prot | P68032;P68133;P63267;P62736 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Actin, Muscle Specific AA range: 2-50
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Monkey, Bovine, Pig, Chicken. Confirm suitability for untested species before use.
Scientific Background
Actin is a a highly conserved cytoskeletal protein, which contains 6 isoformes, of which 4 are muscle tissue specific: α- skeletal muscle actin, α- cardiac muscle actin, α- smooth muscle actin, γ- Smooth muscle actin. Muscle actin is expressed in skeletal muscle, cardiac and smooth muscle, and muscle derived tumors such as leiomyoma and rhabdomyosarcoma. It is usually not expressed in endothelial cells, connective tissue, melanoma, lymphoma and non myogenic sarcoma.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat, Monkey, Bovine, Pig, Chicken. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT-MSA.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
