| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | AFF1;AF4;FEL;MLLT2;PBM1;AF4/FMR2 family member 1;ALL1-fused gene from chromosome 4 protein;Protein AF-4;Protein FEL;Proto-oncogene AF4 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AF4. AA range:1-50 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® AF-4 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against AF-4. It has been validated for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | AF-4 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Immunohistochemistry, Immunofluorescence, Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | AF-4 Polyclonal Antibody detects endogenous levels of AF-4 protein. |
| Molecular Weight | Observed: 130 kDa |
| UniProt / Swiss-Prot | P51825 (Human) | O88573 (Mouse) |
Recommended dilutions: WB 1:500-2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human AF4. AA range:1-50
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in mu
Safety & Handling
This antibody is validated for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA. Recommended dilutions: WB 1:500-2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
