| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | ANP32A;C15orf1;LANP;MAPM;PHAP1;Acidic leucine-rich nuclear phosphoprotein 32 family member A;Acidic nuclear phosphoprotein pp32;Leucine-rich acidic nuclear protein;LANP;Mapmodulin;Potent heat-stable protein phosphatase 2A inhibito |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Purified recombinant fragment of human ANP32A expressed in E. Coli. |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® ANP32A Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against ANP32A. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | ANP32A |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | ANP32A Monoclonal Antibody detects endogenous levels of ANP32A protein. |
| Molecular Weight | Calculated: 29 kDa |
| UniProt / Swiss-Prot | P39687 (Human) | O35381 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;ELISA 1:10000;IF 1:50-200
Immunogen / Antigen
Purified recombinant fragment of human ANP32A expressed in E. Coli.
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
function:Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.,PTM:Phosphorylated on serine residues.,PTM:The N-terminus is blocked.,similarity:Belongs to the ANP32 family.,similarity:Contains 4 LRR (leucine-rich) repeats.,subcellular location:Shuttles between nucleus and cytoplasm.,subunit:Component of the SET complex, which also contains SET, APEX1, HMGB2 and NME1. Directly interacts with SET. Interacts with ATXN1/SCA1. Interacts with MAP1B (By similarity). Interacts with ELAVL1. Part of the INHAT (inhibitor of histone acetyltransferases) complex. Interacts with E4F1.,tissue specificity:Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;ELISA 1:10000;IF 1:50-200
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
