| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | APOA4;Apolipoprotein A-IV;Apo-AIV;ApoA-IV;Apolipoprotein A4 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant fragment of ApoA-IV (aa21-396) expressed in E. Coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® ApoA-IV Mouse Monoclonal Antibody (5F12) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against ApoA-IV. It has been validated for Western Blot, ELISA and reacts with Human.
Validated Applications
| Target | ApoA-IV |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5F12 |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | ApoA-IV Monoclonal Antibody detects endogenous levels of ApoA-IV protein. |
| Molecular Weight | Calculated: 45 kDa |
| UniProt / Swiss-Prot | P06727 (Human) | P06728 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant fragment of ApoA-IV (aa21-396) expressed in E. Coli.
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 5F12.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
