| Field | Specification |
|---|---|
| Mfr No | |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BAX. AA range:80-129 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Bax Rabbit Polyclonal Antibody (WP11252) is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Bax. It has been validated for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA and reacts with Human, Mouse, Rat, Chicken.
Validated Applications
| Target | Bax |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Chicken |
| Applications | Immunohistochemistry, Immunofluorescence, Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | Bax Polyclonal Antibody detects endogenous levels of Bax protein. |
| Molecular Weight | Observed: 22 kDa |
| UniProt / Swiss-Prot | Q07812 (Human) |
Recommended dilutions: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:100-300;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human BAX. AA range:80-129
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Chicken. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by BAX (BCL2 associated X, apoptosis regulator) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for BAX.
Safety & Handling
This antibody is validated for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:100-300;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat, Chicken. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
