| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | BECN1;GT197;Beclin-1;Coiled-coil myosin-like BCL2-interacting protein;Protein GT197 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Purified recombinant fragment of human Beclin-1 expressed in E. Coli. |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Beclin-1 Mouse Monoclonal Antibody (WM0049) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Beclin 1. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA and reacts with Human.
Validated Applications
| Target | Beclin 1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA |
| Purification | Affinity Purified |
| Specificity | Beclin-1 Monoclonal Antibody detects endogenous levels of Beclin-1 protein. |
| Molecular Weight | Calculated: 52 kDa |
| UniProt / Swiss-Prot | Q14457 (Human) | O88597 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;IF 1:50-200
Immunogen / Antigen
Purified recombinant fragment of human Beclin-1 expressed in E. Coli.
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
beclin 1(BECN1) Homo sapiens This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;IF 1:50-200
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
