| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | B cell Oct binding protein 1;B cell specific coactivator OBF 1;B cell specific coactivator OBF1;B-cell-specific coactivator OBF-1;BOB 1;BOB-1;OBF 1;OBF1;OBF1_HUMAN;OCA B;OCA-B;OCAB;OCT binding factor 1;OCT-binding factor 1;POU class 2 associating factor 1;POU domain class 2 associating factor 1;POU domain class 2-associating factor 1;Pou2af1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human BOB.1 AA range: 150-256 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® BOB.1 (ABT-BOB1) Mouse Monoclonal Antibody (WM1287) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against BOB.1. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | BOB.1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT-BOB1 |
| Isotype | IgG2a, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human BOB.1 protein. |
| Molecular Weight | Calculated: 27 kDa | Observed: 35 kDa |
| UniProt / Swiss-Prot | Q16633 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human BOB.1 AA range: 150-256
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
BOB.1 is a transcription activating factor necessary for the formation of follicular germinal center and the production of immunoglobulin. It works together with Oct-2 and is highly expressed in granulocytes of spleen and peripheral blood. It is mainly used for the typing of Hodgkin's lymphoma, mainly nodular lymphocytes. Hodgkin's lymphoma is positive and classical Hodgkin's lymphoma is negative.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT-BOB1.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
