| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | SMARCA4;BAF190A;BRG1;SNF2B;SNF2L4;Transcription activator BRG1;ATP-dependent helicase SMARCA4;BRG1-associated factor 190A;BAF190A;Mitotic growth and transcription activator;Protein BRG-1;Protein brahma homolog 1;SNF2-beta;SWI/S |
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Product Overview
U-Blot® BRG1 Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against BRG1. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Rat.
Validated Applications
| Target | BRG1 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0157R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 185 kDa | Observed: 220 kDa |
| UniProt / Swiss-Prot | P51532 (Human) | Q3TKT4 (Mouse) | Q8K1P7 (Rat) |
Recommended dilutions: IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Immunogen / Antigen
Transcription activator BRG1
Reactivity Notes
Confirmed cross-reactivity: Human, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a member ofThe SWI/SNF family of proteins and is similar toThe brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by alteringThe chromatin structure around those genes.The encoded protein is part ofThe large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulateThe expression ofThe tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Confirmed reactivity: Human, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0157R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
