U-Blot® c-Fos (Phospho Ser362) Rabbit Polyclonal Antibody

SKU:BHA21820941
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-c-Fos antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP9663-50 50 uL
WP9663-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No WP9663
Alternative Names FOS;G0S7;Proto-oncogene c-Fos;Cellular oncogene fos;G0/G1 switch regulatory protein 7
Antigen
  • Proto-oncogene c-Fos
Cellular Localization Nucleus. Endoplasmic reticulum. Cytoplasm, cytosol. In quiescent cells, present in very small amounts in the cytosol. Following induction of cell growth, first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum requires dephosphorylation at Tyr-10 and Tyr-30.
Clonality
  • Polyclonal
Concentration 1 mg/ml
Conjugate
  • Unconjugated
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 2353 (Human) | 14281 (Mouse) | 140675 (Rat)
Host Rabbit
Immunogen The antiserum was produced against synthesized peptide derived from human Fos around the phosphorylation site of Ser362. AA range:331-380
Isotype
  • IgG
Molecular Weight Observed: 62 kDa
Product Type
  • Polyclonal Antibody
  • Primary Antibodies
Reactivity
  • Human
  • Mouse
  • Rat
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Target c-Fos
Antibody

Product Overview

U-Blot® c-Fos (Phospho Ser362) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against c-Fos. Recognizes Phospho. It has been validated for Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target c-Fos
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity Phospho-c-Fos (S362) Polyclonal Antibody detects endogenous levels of c-Fos protein only when phosphorylated at S362.
Molecular Weight Observed: 62 kDa
UniProt / Swiss-Prot P01100 (Human) | P01101 (Mouse) | P12841 (Rat)

Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human Fos around the phosphorylation site of Ser362. AA range:331-380

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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