| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CA9;G250;MN;Carbonic anhydrase 9;Carbonate dehydratase IX;Carbonic anhydrase IX;CA-IX;CAIX;Membrane antigen MN;P54/58N;Renal cell carcinoma-associated antigen G250;RCC-associated antigen G250;pMW1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthetic Peptide of CA IX |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® CA IX Mouse Monoclonal Antibody (12F10) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against CA IX. It has been validated for Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation and reacts with Human.
Validated Applications
| Target | CA IX |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 12F10 |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation |
| Purification | Affinity Purified |
| Specificity | The antibody detects endogenous CA IX proteins. |
| Molecular Weight | Observed: 38-48 kDa |
| UniProt / Swiss-Prot | Q16790 (Human) | Q8VHB5 (Mouse) |
Recommended dilutions: IF 1:50-200;WB 1:3000;IP 1:200;IHC 1:50-300
Immunogen / Antigen
Synthetic Peptide of CA IX
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]
Safety & Handling
This antibody is validated for Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation. Recommended dilutions: IF 1:50-200;WB 1:3000;IP 1:200;IHC 1:50-300
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 12F10.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
