| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Basic calponin;Calponin 1;Calponin 1 basic smooth muscle;Calponin H1;Calponin H1 smooth muscle;Calponin-1;Calponin1;Calponins basic;CNN 1;Cnn1;CNN1_HUMAN;Epididymis secretory protein Li 14;HEL S 14;Sm Calp;SMCC;smooth muscle |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Calponin-1 AA range: 100-200 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Calponin-1 (ABT133) Mouse Monoclonal Antibody (WM1296) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Calponin-1. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | Calponin-1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT133 |
| Isotype | IgG2a, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human and mouse calponin-1 protein. In western blotting of Hela, LnCap and mouse muscle lysates, the antibody can label a 34 kDa band corresponding to calponin-1. |
| Molecular Weight | Calculated: 33 kDa | Observed: 34 kDa |
| UniProt / Swiss-Prot | P51911 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Calponin-1 AA range: 100-200
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
Calponin-1 is a calcium regulatory protein that binds tropomyosin and F-actin. It is a specific protein of smooth muscle cells and has the function of inhibiting the contraction of smooth muscle cells. It is expressed in smooth muscle and myoepithelial cells, as well as myofibroblasts. It is mainly used to study the distribution of myoepithelial cells in smooth muscle tumors and breast tissues.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT133.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
