| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CASP9;MCH6;Caspase-9;CASP-9;Apoptotic protease Mch-6;Apoptotic protease-activating factor 3;APAF-3;ICE-like apoptotic protease 6;ICE-LAP6 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthetic Peptide of Caspase 9 |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Caspase 9 Mouse Monoclonal Antibody (3-20) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Caspase-9. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation and reacts with Human, Mouse, Rat, Chicken.
Validated Applications
| Target | Caspase-9 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3-20 |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Chicken |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation |
| Purification | Affinity Purified |
| Specificity | The antibody detects endogenous Caspase 9 protein. |
| Molecular Weight | Observed: 46 kDa |
| UniProt / Swiss-Prot | P55211 (Human) |
Recommended dilutions: WB 1:1000-5000;IP 1:200;IF 1:200;IHC 1:50-300
Immunogen / Antigen
Synthetic Peptide of Caspase 9
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Chicken. Confirm suitability for untested species before use.
Scientific Background
CASP9 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Caspase 9 can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. Caspase 9 is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation. Recommended dilutions: WB 1:1000-5000;IP 1:200;IF 1:200;IHC 1:50-300
Confirmed reactivity: Human, Mouse, Rat, Chicken. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 3-20.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
