| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | APY;ATOPY;B lymphocyte antigen CD20;B Lymphocyte Cell Surface Antigen B1;B-lymphocyte antigen CD20;B-lymphocyte cell-surface antigen B1;B-lymphocyte surface antigen B1;B1;Bp 35;Bp35;CD 20;CD20;CD20 antigen;CD20 receptor;CD20_HUMAN;CVID 5;CVID5;Fc epsilon receptor I beta chain;Fc Fragment of IgE high affinity I receptor for beta polypeptide;FCER1B;High affinity immunoglobulin epsilon receptor subunit beta;IgE Fc receptor subunit beta;IGEL;IGER;IGHER;LEU 16;Leu-16;LEU16;leukocyte surface antigen Leu 16;Leukocyte surface antigen Leu-16;Ly44;Membrane spanning 4 domains A1;Membrane spanning 4 domains subfamily A member 2;membrane-spanning 4-domains A1;Membrane-spanning 4-domains subfamily A member 1;MGC3969;MS4A1;MS4A2;S7 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human CD20 AA range: 200-297 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® CD20 (ABT065) Mouse Monoclonal Antibody (WM1312) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against CD20. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | CD20 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT065 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human CD20 protein. |
| Molecular Weight | Calculated: 33 kDa | Observed: 35 kDa |
| UniProt / Swiss-Prot | P11836 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human CD20 AA range: 200-297
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
CD20 is a B cell specific membrane protein, which is mainly distributed in B cells, pre-B cells, post-B cells and B cells before plasma cells. CD20 antibody generally does not cross react with T cells, and has good cell specificity for B-cell lymphoma and acute and chronic lymphocytic leukemia. Therefore, CD20 is the most commonly used B-cell marker in the classification of malignant lymphoma, especially T or B-cell lymphoma.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT065.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.