| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 4930549J05Rik;A430104F18Rik;AW552088;Cd247;CD247 antigen;CD247 antigen, zeta subunit;CD247 molecule;CD3;CD3 antigen, delta subunit;CD3 delta;CD3 epsilon;CD3 eta;CD3 gamma;CD3 molecule delta polypeptide;CD3 molecule, epsilon polypeptide;CD3 molecule, gamma polypeptide;CD3 zeta;CD3-DELTA;CD3d;CD3D antigen delta polypeptide;CD3d antigen, delta polypeptide;TiT3 complex;CD3d molecule delta;CD3d molecule delta CD3 TCR complex;CD3d molecule, delta;CD3-TCR complex;CD3D_HUMAN;CD3E;CD3e antigen;CD3E antigen epsilon polypeptide;CD3e antigen, epsilon polypeptide;TiT3 complex;CD3e molecule epsilon;CD3e molecule epsilon CD3 TCR complex;CD3e molecule, epsilon;CD3-TCR complex;CD3epsilon;CD3G;CD3g antigen;CD3G antigen gamma polypeptide;CD3g antigen, gamma polypeptide;TiT3 complex;CD3g molecule gamma;CD3g molecule gamma CD3 TCR complex;CD3g molecule, gamma;CD3-TCR complex;CD3H;CD3Q;CD3Z;CD3zeta;Ctg3;FLJ17620;FLJ17664;FLJ18683;FLJ79544;FLJ94613;IMD19;Leu-4;MGC138597;OKT3, delta chain;OTTHUMP00000032544;T cell receptor;T cell receptor T3 delta chain;T cell receptor T3 gamma chain;T cell receptor T3 zeta chain;T cell receptor zeta chain;T cell surface antigen T3/Leu 4 epsilon chain;T cell surface glycoprotein CD3;T cell surface glycoprotein CD3 delta chain;T cell surface glycoprotein CD3 epsilon chain;T cell surface glycoprotein CD3 gamma chain;T cell surface glycoprotein CD3 zeta chain;T-cell antigen receptor complex, delta subunit of T3;T-cell antigen receptor complex, epsilon subunit of T3;T-cell antigen receptor complex, gamma subunit of T3;T-cell antigen receptor complex, zeta subunit of CD3;T-cell receptor T3 delta chain;T-cell receptor T3 gamma chain;T-cell surface antigen T3/Leu-4 epsilon chain;T-cell surface glycoprotein CD3 delta chain;T-cell surface glycoprotein CD3 epsilon chain;T-cell surface glycoprotein CD3 gamma chain;T3;T3d;T3e;T3g;T3z;TCRE;TCRk;Tcrz;TCRzeta |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human CD3, CD3E AA range: 1-100 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® CD3 (ABT295) Mouse Monoclonal Antibody (WM1320) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against CD3E. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | CD3E |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT295 |
| Isotype | IgG2a, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human CD3e protein, and shows no reaction with CD3d or CD3g. In western blotting of Jurkat cell lysate, the antibody can label a 23 kDa band corresponding to CD3e. |
| Molecular Weight | Calculated: 23 kDa | Observed: 23 kDa |
| UniProt / Swiss-Prot | P07766 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human CD3, CD3E AA range: 1-100
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
CD3 protein contains ε、γ、δ and ζ four subunits, including extracellular, transmembrane and intracellular domains. CD3 is a T cell marker, which is not expressed by B cells. CD3 complex mediates TCR antigen recognition, leading to T cell proliferation and cytokine release. It is mainly used for the diagnosis of T-cell lymphoma, with high specificity but low sensitivity. In order to improve the accuracy of diagnosis, it is usually combined with CD45RO in clinical application.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT295.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
