| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C3 binding protein;C3b/C4b receptor;C3BR;C4BR;CD 35;CD35;CD35 antigen;complement component;3b/4b;receptor 1;Knops blood group;complement component;3b/4b;receptor 1 including Knops blood group system;Complement component receptor 1;Complement receptor 1;Complement receptor type 1;CR 1;CR1;CR1_HUMAN;KN;Knops blood group antigen |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human CD35 AA range: 300-400 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® CD35 (ABT-CD35) Mouse Monoclonal Antibody (WM1383) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against CD35. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | CD35 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT-CD35 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human CD35 protein. |
| Molecular Weight | Calculated: 223 kDa | Observed: 250 kDa |
| UniProt / Swiss-Prot | P17927 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human CD35 AA range: 300-400
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
CD35, also known as complement receptor 1, is a membrane penetrating glycoprotein with a molecular weight of 210-220 kDa, which can bind to complement C3b and C4b. CD35 is expressed in follicular dendritic cells, mature B lymphocytes, monocytes, erythrocytes, glomerular podocytes and some T cells. It is mainly used for the study of follicular dendritic cells and their derived tumors.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT-CD35.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
