| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CD44 antigen;CDw44;Epican;Extracellular matrix receptor III;ECMR-III;GP90 lymphocyte homing/adhesion receptor;HUTCH-I;Heparan sulfate proteoglycan;Hermes antigen;Hyaluronate receptor;Phagocytic glycoprotein 1;PGP-1;Phagocytic glycoprotein I;PGP-I;CD antigen CD44 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human CD44 AA range: 100-200 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® CD44 (ABT476) Mouse Monoclonal Antibody (WM1501) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against CD44. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | CD44 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT476 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human CD44 protein. In western blotting of Hela cell lysate, the antibody can label a 81 kDa band corresponding to CD44. |
| Molecular Weight | Calculated: 81 kDa | Observed: 81 kDa |
| UniProt / Swiss-Prot | P16070 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human CD44 AA range: 100-200
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
CD44 is a cell surface receptor, which belongs to the family of cell adhesion molecules that mediate cell-to-cell and cell-to-matrix adhesion, and interacts with cytoskeletal proteins. CD44 is widely expressed in hematopoietic cells and mesodermal cells and is a marker of these cells. Studies have shown that the loss of CD44 expression is related to invasion, metastasis and progression of lung cancer, prostate cancer, breast cancer, ovarian cancer and malignant melanoma.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT476.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
